The athletic achievement by the 34-year-old Kenyan is considered the holy grail of running and will secure his legendary status.
After crossing the line a beaming Kipchoge continued running and waving at well-wishers who had gathered to greet him.
The remarkable run in Vienna by the four-time winner of the London marathon follows six months of gruelling preparation.
Eliud Kipchoge was supported by a 41-strong team of rotating pacesetters
He missed out on the elusive target by just 25 seconds in his first attempt in Monza two years ago.
Although the landmark feat will not stand as an official record because it was not in open competition and used a 41-strong team of rotating pacesetters, it marks a major milestone in running and world sport.
Speaking during the attempt in Vienna, Britain’s four-time Tour de France champion Chris Froome expressed confidence Kipchoge would succeed.
Froome told Sky News: “I feel just as nervous on the other side of the barriers for him but he looks so calm, so composed.
“I’ve got all the confidence he is going to pull this off. He’s just gliding along. It’s incredible to watch and witness this first hand.
“The crowds are great, lots of Kenyans and his fellow countrymen being here will give him that added motivation and drive to get this done.
“If he pulls it off it would just show limits can be pushed, limits can be broken.”
