Miller was due to fight IBF, WBA, and WBO champion Joshua before an “adverse finding” from a sample taken on March 20, threw his participation in the bout into doubt.
After being denied a licence to fight by the New York State Athletic Commission, the American boxer said he had never knowingly taken a banned substance, adding that he intended to appeal against the decision to revoke his licence.
Joshua’s promoter, Eddie Hearn, has now revealed that he has learnt of a second alleged doping violation by Miller, meaning that fight is now all but dead in the water.
“We have now received news from VADA that Jarrell Miller has now failed a second separate test for a further substance. AJ’s June 1 opponent will be announced next week – if you don’t think you can beat him clean then delete my number!” Hearn wrote on Twitter.
A statement released to Sky Sports News later on Friday evening from Jarrell Miller’s promoters, Salita Promotions, read: “Salita Promotions and Greg Cohen Promotions are very disappointed in these findings and feel awful that fight fans will be deprived of what would have been a great fight.
“It’s a terrible outcome and unfortunate situation for all parties involved.”